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Hairpin precursor miRNA of approximately 150 nucleotides is cloned into lentiviral or non-viral vectors for delivery in virtually all cell types.
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MicroRNA: mmu-miR-6238 Accession Number: MIMAT0024859 Mature Sequence: UUAUUAGUCAGUGGAGGAAAUG mmu-miR-6238 are small non-coding RNAs of 20-22 nucleotides, typically excised from 60-110 nucleotide foldback RNA precursor structures. miRNAs are involved in crucial biological processes, including development, differentiation,
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Image Search Results
Journal: The EMBO Journal
Article Title: Single‐cell transcriptomics of suprachiasmatic nuclei reveal a Prokineticin‐driven circadian network
doi: 10.15252/embj.2021108614
Figure Lengend Snippet:
Article Snippet: Recombinant Prok2 was purchased from Sigma Aldrich (#SRP3146) and
Techniques: Plasmid Preparation, Recombinant, Modification, RNAscope, Multiplex Assay, Software
Journal: American Journal of Cancer Research
Article Title: δ-Catenin promotes cell migration and invasion via Bcl-2-regulated suppression of autophagy in prostate cancer cells
doi:
Figure Lengend Snippet: δ-Catenin siRNA disrupted migration and invasion ability and increased autophagy in RV/δ cells. RV/δ cells were transfected with control siRNA, δ-catenin siRNA#1, and δ-catenin siRNA#2. A. The wound-healing scratch assay was used to detect the cell migration ability of each group. Quantitative analysis of cell migration in 3 independent experiments (n=3). Magnification: 50×. B. The Transwell assay was used to analyze the invasion ability of each group. Quantitative analysis of cell invasion in 3 independent experiments (n=3). Magnification: 100×. C. Western blot analysis of LC3, β-catenin, and Bcl-2 protein levels in each group. The relative quantification of protein levels was performed using ImageJ software. Data are shown as means ± SD of 3 independent experiments. *P<0.05, **P<0.01, and ***P<0.001.
Article Snippet: Rapamycin (HY-10219) and bafilomycin A1 (HY-100558) were purchased from MedChemExpress (USA). δ-Catenin siRNA#1 (145777) and δ-catenin siRNA#2 (145778) were purchased from Thermo Fisher Scientific. β-Catenin siRNA#1 (6225) and
Techniques: Migration, Transfection, Control, Wound Healing Assay, Transwell Assay, Western Blot, Quantitative Proteomics, Software
Journal: American Journal of Cancer Research
Article Title: δ-Catenin promotes cell migration and invasion via Bcl-2-regulated suppression of autophagy in prostate cancer cells
doi:
Figure Lengend Snippet: δ-Catenin downregulated autophagy via β-catenin transcriptional regulation of Bcl-2. A. 22RV1, RV/C, and RV/δ cells treated with 0, 50, 100, or 200 nM rapamycin. Western blots were used to analyze β-catenin protein levels in each group. B. RV/C cells were transfected with GFP control. RV/δ cells were transfected with GFP control and GFP-δ-catenin. Western blot analysis of LC3 and Bcl-2 protein levels in each group. C. QRT-PCR analysis of Bcl-2 mRNA levels. D. RV/C and RV/δ cells treated with β-catenin siRNA and control siRNA. Western blot analysis of LC3, β-catenin, Beclin1, and Bcl-2 protein levels in each group. Data are shown as means ± SD of 3 independent experiments. *P<0.05, **P<0.01, and ***P<0.001.
Article Snippet: Rapamycin (HY-10219) and bafilomycin A1 (HY-100558) were purchased from MedChemExpress (USA). δ-Catenin siRNA#1 (145777) and δ-catenin siRNA#2 (145778) were purchased from Thermo Fisher Scientific. β-Catenin siRNA#1 (6225) and
Techniques: Western Blot, Transfection, Control, Quantitative RT-PCR